The detection of bacterial contamination of water,
pharmaceutical products etc. is of great importance, and is
most conveniently performed by the detection of bacterial
ATP (Adenosine TriPhosphate) using the luciferin-luciferase
bioluminescence system. This system uses unstable and
expensive reagents, and emits transient light signals. In
this study an FIA (Flow Injection Analysis) system was set
up to monitor the light signal produced by the reaction.
Using a luminometric detector (a liquid scintillation
counter) with the FIA system, the reaction length, sample
volume, flow rates, pH etc. were investigated. [Continues.]
A doctoral thesis submitted in partial fulfilment of the requirements for the award of Doctor of Philosophy at Loughborough University.