A gas chromatographic method for the routine analysis of volatile and
semi-volatile sulphur compounds in beer was developed. Various selective
and specific detectors, capillary columns and methods of sample preparation
were compared. The combination offering the best sensitivity and stability
consisted of solid phase microextraction (SPME) as the sample preparation
step; a combined polar/non-polar chromatographic column; and a pulsed
flame photometric detector (PFPD). All parameters were optimised to
achieve maximum sensitivity. The system was linear for the range of sulphur
compound concentrations found in beer, and displayed good reproducibility.
The calibrated SPME-GC-PFPD system was used to analyse several
different varieties of beer from a range of breweries, and to investigate the
change in the concentrations of sulphur compounds when beer is subjected
A Doctoral Thesis. Submitted in partial fulfilment of the requirements for the award of Doctor of Philosophy at Loughborough University.