Thesis-1991-AbuElteen.pdf (11.16 MB)
Effect of sub-inhibitory concentrations of antifungal drugs on adherence of Candida species
thesis
posted on 2018-05-30, 15:18 authored by Khalid H.A. Abu-ElteenThe adherence of three Candida species to human buccal
epithelial cells (BECs) following treatment of the yeast with sub-inhibitory
concentrations of amphotericin B, nystatin, miconazole
nitrate, 5-fluorocytosine, octenidine and pirtenidine was investigated in vitro. Pre-incubation of C. albicans (two strains), C.
tropicalis or C.kefyr with these antifungal drugs inhibited their
adherence to varying degrees (reduction between 17% and 78% of
the control value). Pre-treatment of yeast for a short period
(l hr) had less effect on adhesion than pre-treatment for a long
period (24 hr). Furthermore, treating C. albicans with a combination
of amphotericin B plus 5-fluorocytosine, both at 1/8 MIC
level, led to stronger adherence inhibition than that obtained for
yeast pre-treated with either one alone at 1/4 MIC levels. In
addition, the pre-treatment of either Candida or BECs or both
types of cells with the drugs reduced adherence, the reduction
being greatest when both types of cells were pre-treated. No
difference in adherence between stationary or exponential phase
yeast to BEC was observed and the drugs were effective in
reducing the adherence of cells from either growth phase. [Continues.]
History
School
- Science
Department
- Chemistry
Publisher
© Khalid Hussein Abu-ElteenPublisher statement
This work is made available according to the conditions of the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) licence. Full details of this licence are available at: https://creativecommons.org/licenses/by-nc-nd/4.0/Publication date
1991Notes
A Doctoral Thesis. Submitted in partial fulfilment of the requirements for the award of Doctor of Philosophy at Loughborough University.Language
- en