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|Title: ||The determination of salivary oxypurines before and after exercise by combined liquid chromatography-field asymmetric waveform ion mobility spectrometry-time-of-flight mass spectrometry|
|Authors: ||Arthur, Kayleigh L.|
Wilson, Lynsey S.
Turner, Matthew A.
Lindley, Martin R.
Reynolds, James C.
Creaser, Colin S.
Differential mobility spectrometry
|Issue Date: ||2018|
|Publisher: ||© Springer|
|Citation: ||ARTHUR, K.L. ...et al., 2018. The determination of salivary oxypurines before and after exercise by combined liquid chromatography-field asymmetric waveform ion mobility spectrometry-time-of-flight mass spectrometry. International Journal for Ion Mobility Spectrometry, 21(3), pp 87–95.|
|Abstract: ||© 2018 Springer-Verlag GmbH Germany, part of Springer Nature A method combining field asymmetric waveform ion mobility spectrometry with liquid chromatography-mass spectrometry (LC-FAIMS-MS) has been developed for the analysis of the oxypurine compounds hypoxanthine (HX) and xanthine (XA) in saliva. Separation of the oxypurines from interfering matrix components was investigated using FAIMS-MS. The selected FAIMS parameters were then applied to the rapid LC-FAIMS-MS analysis of HX and XA using a short chromatographic separation method (7 min). A comparison of the LC-MS method with and without FAIMS applied, resulted in improved discrimination from saliva matrix interferences and improved chromatographic peak integration for both HX and XA using a FAIMS separation. A quantitative evaluation of the LC-FAIMS-MS method was performed giving limits of detection of 2.0 ng mL −1 for HX and 1.8 ng mL −1 for XA, and limits of quantification of 6.6 ng mL −1 for HX and 6.0 ng mL −1 for XA. The developed LC-FAIMS-MS method was applied to the targeted analysis of the oxypurine metabolites in saliva collected from healthy male athletes (n = 11) before and after exercise designed to induce oxidative stress; post-exercise collection time-points included immediately after exercise, one hour and twenty-four hours’ post-exercise. The salivary concentrations of both HX and XA were lower after physical exercise, compared to the pre-exercise (rest) concentrations and returned to approximately pre-exercise levels after twenty-four hours. The method reported has the potential for monitoring the salivary oxypurines, HX and XA, as biomarkers of oxidative stress and in other clinical applications.|
|Description: ||This paper is in closed access until 19th May 2019.|
|Sponsor: ||This work was supported by Owlstone Ltd. and
|Version: ||Accepted for publication|
|Publisher Link: ||https://doi.org/10.1007/s12127-018-0232-4|
|Related Resource: ||https://doi.org/10.17028/rd.lboro.6551114|
|Appears in Collections:||Closed Access (Sport, Exercise and Health Sciences)|
Closed Access (Chemistry)
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