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Title: Engineering thermoresponsive phase separated vesicles formed: Via emulsion phase transfer as a content-release platform
Authors: Karamdad, Kaiser
Hindley, James W.
Bolognesi, Guido
Friddin, Mark S.
Law, Robert V.
Brooks, Nicholas J.
Ces, Oscar
Elani, Yuval
Issue Date: 2018
Publisher: Royal Society of Chemistry
Citation: KARAMDAD, K. ...et al., 2018. Engineering thermoresponsive phase separated vesicles formed: Via emulsion phase transfer as a content-release platform. Chemical Science, 9(21), pp. 4851-4858.
Abstract: © 2018 The Royal Society of Chemistry. Giant unilamellar vesicles (GUVs) are a well-established tool for the study of membrane biophysics and are increasingly used as artificial cell models and functional units in biotechnology. This trend is driven by the development of emulsion-based generation methods such as Emulsion Phase Transfer (EPT), which facilitates the encapsulation of almost any water-soluble compounds (including biomolecules) regardless of size or charge, is compatible with droplet microfluidics, and allows GUVs with asymmetric bilayers to be assembled. However, the ability to control the composition of membranes formed via EPT remains an open question; this is key as composition gives rise to an array of biophysical phenomena which can be used to add functionality to membranes. Here, we evaluate the use of GUVs constructed via this method as a platform for phase behaviour studies and take advantage of composition-dependent features to engineer thermally-responsive GUVs. For the first time, we generate ternary GUVs (DOPC/DPPC/cholesterol) using EPT, and by compensating for the lower cholesterol incorporation efficiencies, show that these possess the full range of phase behaviour displayed by electroformed GUVs. As a demonstration of the fine control afforded by this approach, we demonstrate release of dye and peptide cargo when ternary GUVs are heated through the immiscibility transition temperature, and show that release temperature can be tuned by changing vesicle composition. We show that GUVs can be individually addressed and release triggered using a laser beam. Our findings validate EPT as a suitable method for generating phase separated vesicles and provide a valuable proof-of-concept for engineering content release functionality into individually addressable vesicles, which could have a host of applications in the development of smart synthetic biosystems.
Description: This is an Open Access Article. It is published by Royal Society of Chemistry under the Creative Commons Attribution 3.0 Unported Licence (CC BY). Full details of this licence are available at: http://creativecommons.org/licenses/by/3.0/
Sponsor: This work was supported by the EPSRC via grants EP/J017566/1, EP/K038648/1, EP/K503733/1, EPSRC Fellowship EP/N016998/1 awarded to YE and an EPSRC Centre for Doctoral Training Studentship from the Institute of Chemical Biology (Imperial College London) awarded to JWH.
Version: Published
DOI: 10.1039/c7sc04309k
URI: https://dspace.lboro.ac.uk/2134/33605
Publisher Link: https://doi.org/10.1039/c7sc04309k
ISSN: 2041-6520
Appears in Collections:Published Articles (Chemical Engineering)

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