Thesis-1973-Gretton.pdf (2.44 MB)
Studies of aromatic hydroxylation
thesis
posted on 2018-07-09, 14:27 authored by William R. GrettonThe conversion of [4-3H]- and [4-3H; 3,5-2H2]-phenyl-alanine
into tyrosine by cultures of Pseudomonas sp. (NCIB
9289) has been studied. Fromm a consideration of the degree
of retention of tritium observed during the hydroxylation
of the two precursors a kinetic isotope effect, kD/kT =
2.8 ± 0.1 (kH/kD = 10 ± 1), has been calculated for the
final aromatisation process following the NIH shift. The
relevance of this result to the overall reaction pathway
is discussed in the light of similar data derived from
possible chemical models for the biological process. [Continues.]
Funding
Science Research Council.
History
School
- Science
Department
- Chemistry
Publisher
© William Roy GrettonPublisher statement
This work is made available according to the conditions of the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) licence. Full details of this licence are available at: https://creativecommons.org/licenses/by-nc-nd/4.0/Publication date
1973Notes
A Doctoral Thesis. Submitted in partial fulfilment of the requirements for the award of Doctor of Philosophy at Loughborough University.Language
- en
