Thesis-2000-Sarpara.pdf (31.11 MB)
Fluorigenic enzyme assays using long-wavelength substrates
thesis
posted on 2018-08-31, 15:29 authored by G. Hussain SarparaInvestigations into the use of long-wavelength fluorigenic substrates, which are
non- or weakly fluorescent but are converted by an appropriate enzyme to a
highly fluorescent product, have been carried out in order to determine several
different enzymes and their inhibitors.
Certain nonfluorescent compounds based on long-wavelength xanthenes and
oxazines dyes were synthesised. Amongst these, naphthofluorescein diphosphate
and naphthofluorescein mono-phosphate has been demonstrated
to be new fluorigenic substrates for alkaline phosphatase. The hydrolysis
product of these substrates can be used for trace analyses using diode laser
based fluorescence detection. [Continues.]
Funding
Pakistan, Ministry of Education.
History
School
- Science
Department
- Chemistry
Publisher
© Ghulam Hussain SarparaPublisher statement
This work is made available according to the conditions of the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) licence. Full details of this licence are available at: https://creativecommons.org/licenses/by-nc-nd/4.0/Publication date
2000Notes
A Doctoral Thesis. Submitted in partial fulfilment of the requirements for the award of Doctor of Philosophy at Loughborough University.Language
- en