acsomega.pdf (3.25 MB)
Controlled arrangement of neuronal cells on surfaces functionalized with micro-patterned polymer brushes
journal contribution
posted on 2018-09-28, 14:44 authored by Maria M. Pardo-Figuerez, Neil MartinNeil Martin, Darren J. Player, Paul RoachPaul Roach, Steven ChristieSteven Christie, Andrew CapelAndrew Capel, Mark LewisMark LewisConventional in vitro cultures are useful to represent simplistic neuronal behaviour,
however the lack of organisation results in random neurite spreading. To overcome this
problem, control over the directionality of SH-SY5Y cells was attained, utilising photolithography to pattern the cell-repulsive anionic brush poly(potassium 3-sulfopropyl
methacrylate) (PKSPMA) into tracks of 20, 40, 80 and 100 µm width. This data validates the
use of PKSPMA brush coatings for long-term culture of SH-SY5Y cells, as well as providing
a methodology by which the precise deposition of PKSPMA can be utilised to achieve targeted
control over SH-SY5Y cells. Specifically, PKSPMA brush patterns prevented cell attachment,
allowing SH-SY5Y cells to grow only on the non-coated glass (gaps of 20, 50, 75 and 100 µm
width) at different cell densities (5000, 10000 and 15000 cells/cm2). This research
demonstrates the importance of achieving cell directionality in vitro, whilst these simplistic
models could provide new platforms to study complex neuron-neuron interactions.
Funding
This research was supported by EPSRC EP/L02067X/1
History
School
- Sport, Exercise and Health Sciences
Published in
ACS OmegaCitation
PARDO-FIGUEREZ, M.M. ... et al., 2018. Controlled arrangement of neuronal cells on surfaces functionalized with micro-patterned polymer brushes. ACS Omega, 3 (10), pp.12383–12391Publisher
American Chemical SocietyVersion
- AM (Accepted Manuscript)
Publisher statement
This work is made available according to the conditions of the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) licence. Full details of this licence are available at: https://creativecommons.org/licenses/by-nc-nd/4.0/Acceptance date
2018-09-17Publication date
2018-10-01Notes
This is an open access article published under a Creative Commons Attribution (CC-BY) License, which permits unrestricted use, distribution and reproduction in any medium, provided the author and source are cited.ISSN
2470-1343Publisher version
Language
- en