SDR review paper WO. (GD)docx (accepted) final.pdf (434.93 kB)
Insight into the large-scale upstream fermentation environment using scaled-down models
journal contribution
posted on 2018-10-01, 09:59 authored by Williams Olughu, Gurjot Deepika, Christopher J. Hewitt, Chris RiellyChris RiellyScaled‐down models are small‐scale bioreactors, used to mimic the chemical (pH, nutrient and dissolved oxygen) and physical (pressure, viscosity and temperature) gradients known to occur in the large‐scale fermenter. Conventionally, before scaling up any bioprocess, small‐scale bioreactors are used for strain selection, characterisation and optimisation. The typical small‐scale environment is homogenous, hence all the cells held within the small‐scale bioreactor can be assumed to experience the same condition at any point in time. However, for the large‐scale bioreactor, this is not the case, due to its inhomogeneous environment. Three different scaled‐down models are reviewed here, and the results suggest that a bacterium responds to changes in its environment rapidly and the magnitude of response to environmental oscillations is organism‐specific. The reaction and adaption of a bacterium to an inhomogeneous environment in most cases result in productivity and quality losses. This review concludes that consideration of fermentation gradients should be paramount when researchers screen for high yielding mutants in bioprocess development and doing this would help mitigate performance loss on scale‐up.
Funding
Engineering and Physical Sciences Research Council. Grant Number: 1472593
History
School
- Aeronautical, Automotive, Chemical and Materials Engineering
Department
- Chemical Engineering
Published in
Journal of Chemical Technology and BiotechnologyCitation
OLUGHU, W.C. ... et al, 2018. Insight into the large-scale upstream fermentation environment using scaled-down models. Journal of Chemical Technology and Biotechnology, 94 (3), pp.647-657.Publisher
Wiley © Society of Chemical IndustryVersion
- AM (Accepted Manuscript)
Publisher statement
This is the peer reviewed version of the following article: OLUGHU, W.C. ... et al, 2018. Insight into the large-scale upstream fermentation environment using scaled-down models. Journal of Chemical Technology and Biotechnology, 94 (3), pp.647-657, which has been published in final form at https://doi.org/10.1002/jctb.5804. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.Acceptance date
2018-08-22Publication date
2018ISSN
0268-2575eISSN
1097-4660Publisher version
Language
- en