JPFSM7(5) 261-267 (2018).pdf (1.14 MB)
Effects of eicosapentaenoic acid intake on denervation-induced mitochondrial adaptation in mouse skeletal muscle
journal contribution
posted on 2018-10-31, 10:24 authored by Kohei Takeda, Yu Kitaoka, Koichi Watanabe, Shumpei Miyakawa, Martin Lindley, Tohru TakemasaEicosapentaenoic acid (EPA) is an omega-3 polyunsaturated fatty acid that is abundant in fish oil, and has anti-inflammatory or anti-obesity effects. However, the effects of EPA supplementation on mitochondrial content and dynamics (fusion and fission) in skeletal muscle has not been elucidated. We investigated the effects of EPA intake for 4 weeks on denervation-induced mitochondrial adaptation in mice skeletal muscle. ICR mice (male, 8 weeks old) were daily administrated olive oil (control) or EPA at a dose of 300 mg/kg body weight by gavage for 4 weeks. After 2 weeks of oil intake, mice underwent unilateral sciatic nerve transection surgery. The hindlimb without surgery served as the sham-operated control. Body and skeletal muscle weights did not differ between the control and the EPA groups. Peroxisome proliferator-activated receptor gamma coactivator 1 alpha (PGC-1α) and oxidative phosphorylation (OXPHOS) proteins were significantly decreased by denervation surgery. Denervation also induced the reduction of mitochondrial fusion molecules and the increase of mitochondrial fission molecules. Expression levels of PGC-1α and mitochondrial respiration protein MTCO1 were higher in the EPA group than in the control (olive oil) group. In addition, the EPA group contained higher levels of mitochondrial fusion protein OPA1. Our results suggest that EPA intake prevents the reduction of mitochondrial content and fusion proteins in denervated skeletal muscle.
History
School
- Sport, Exercise and Health Sciences
Published in
Japanese Journal of Physical Fitness and Sports MedicineVolume
7Issue
5Pages
261 - 267 (7)Citation
TAKEDA, K. ... et al, 2018. Effects of eicosapentaenoic acid intake on denervation-induced mitochondrial adaptation in mouse skeletal muscle. The Journal of Physical Fitness and Sports Medicine, 7 (5), pp.261-267.Publisher
© The Japanese Society of Physical Fitness and Sports MedicineVersion
- VoR (Version of Record)
Publisher statement
This work is made available according to the conditions of the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) licence. Full details of this licence are available at: https://creativecommons.org/licenses/by-nc-nd/4.0/Acceptance date
2018-06-06Publication date
2018-09-25Notes
This is an Open Access Article. It is published by The Japanese Society of Physical Fitness and Sports Medicine under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International Licence (CC BY-NC-ND). Full details of this licence are available at: http://creativecommons.org/licenses/by/4.0/ISSN
2186-8131eISSN
2186-8123Publisher version
Language
- en