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Title: Direct manipulation of liquid ordered lipid membrane domains using optical traps
Authors: Friddin, Mark S.
Bolognesi, Guido
Salehi-Reyhani, Ali
Ces, Oscar
Elani, Yuval
Issue Date: 2019
Publisher: © the Authors. Published by Springer Nature
Citation: FRIDDIN, M.S. ... et al., 2019. Direct manipulation of liquid ordered lipid membrane domains using optical traps. Communications Chemistry, 2: 6.
Abstract: Multicomponent lipid bilayers can give rise to coexisting liquid domains that are thought to influence a host of cellular activities. There currently exists no method to directly manipulate such domains, hampering our understanding of their significance. Here we report a system that allows individual liquid ordered domains that exist in a liquid disordered matrix to be directly manipulated using optical tweezers. This allows us to drag domains across the membrane surface of giant vesicles that are adhered to a glass surface, enabling domain location to be defined with spatiotemporal control. We can also use the laser to select individual vesicles in a population to undergo mixing/demixing by locally heating the membrane through the miscibility transition, demonstrating a further layer of control. This technology has potential as a tool to shed light on domain biophysics, on their role in biology, and in sculpting membrane assemblies with user-defined membrane patterning.
Description: This is an Open Access Article. It is published bySpringer under the Creative Commons Attribution 4.0 Unported Licence (CC BY). Full details of this licence are available at: http://creativecommons.org/licenses/by/4.0/
Sponsor: This work was supported by the EPSRC via grant EP/J017566/1 and by EPSRC Fellowship EP/N016998/1 awarded to Y.E.
Version: Published
DOI: 10.1038/s42004-018-0101-4
URI: https://dspace.lboro.ac.uk/2134/36850
Publisher Link: https://doi.org/10.1038/s42004-018-0101-4
Appears in Collections:Published Articles (Chemical Engineering)

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