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Anticalcification potential of heparin on hydroxyapatite seeds using a constant composition in vitro model
journal contribution
posted on 2019-03-01, 10:04 authored by Adel Badria, Petros Koutsoukos, Cristian D'Alessandro, Sotiris KorossisSotiris Korossis, Dimosthenis MavrilasCalcification is among the principal causes of biological heart valve substitute failure. Glycosaminoglcans
(GAGs) are negatively charged molecules, possessing anticoagulation and anti-inflammatory activity that make
them a potential solution against calcification. In the present work, the anticalcification potential of heparin was
investigated under constant supersaturation conditions with respect to hydroxyapatite (Ca5(PO4)3OH; HAP).
Heparin concentration in the supersaturated solutions was in the range between 0.25 and 3 ppm, at pH 7.40 and
37 °C. Heparin showed inhibitory activity, which was attributed to adsorption at the active crystal growth
centres. Heparin concentration as low as ca. 50 μM, reduced the rate of HAP crystal growth by more than 80%,
while further increase (up to 200 μM) failed to completely inhibit the process beyond 90%. Heparin uptake
studies at equilibrium conditions and analysis of the kinetics data in the presence of heparin, strongly suggest
that the inhibition is due to the adsorption of heparin onto the HAP crystals, which resulted in the poisoning of
the active growth sites.
Funding
This research was supported by the People Programme (Marie Curie Actions) of the European Union’s Seventh Framework Program FP7/ 2007-2013/ under REA grant agreement n° 317512.
History
School
- Mechanical, Electrical and Manufacturing Engineering
Published in
Journal of Crystal GrowthVolume
498Pages
399 - 404Citation
BADRIA, A. ... et al., 2018. Anticalcification potential of heparin on hydroxyapatite seeds using a constant composition in vitro model. Journal of Crystal Growth, 498, pp. 399 - 404.Publisher
© Elsevier BVVersion
- VoR (Version of Record)
Publisher statement
This work is made available according to the conditions of the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) licence. Full details of this licence are available at: https://creativecommons.org/licenses/by-nc-nd/4.0/Acceptance date
2018-07-10Publication date
2018-07-11Notes
This paper is in closed access.ISSN
0022-0248Publisher version
Language
- en