Loughborough University
Browse
Development of Tissue Engineered Skeletal Muscle Manufacturing Variables.pdf (378.99 kB)

Development of tissue-engineered skeletal muscle manufacturing variables

Download (378.99 kB)
journal contribution
posted on 2019-07-08, 12:31 authored by Nick Wragg, Darren J. Player, Neil MartinNeil Martin, Yang LiuYang Liu, Mark LewisMark Lewis
Three-dimensional tissue-engineered structures enable more representative determination of novel drug or material effects on tissue than traditional monolayer cell cultures. This study sought to better understand how key manufacturing variables affect the myotube characteristics of a skeletal muscle model toward reducing resource use and to develop an understanding of scaling on model consistency. C2C12 murine myoblasts were seeded in a tethered collagen scaffold from which directional myotubes form in response to lines of tension and a change in medium. Collagen polymerizing area length-to-width ratios greater than one were found to reduced cell–matrix attachment and remodeling forces significantly (p <.05) correlating to a reduction in cell fusion potential. Following this, utilizing a factorial design of experiment, 4 million C2C12s/ml, with a polymerizing area width 150% of the anchor point, produced the most favorable myotube characteristics and dramatically reduced the incidence of rupture. Scaled constructs showed no significant differences when compared to larger models. Approximately 20 myotubes with a variation in the alignment of <25° in the central region were consistently observed in the final models. This demonstrates the influence of initial manufacturing variables on tissue formation and has produced a benchmark model for consistent production across scaled constructs for future optimization and as a potential cost-effective preclinical testbed.

History

School

  • Mechanical, Electrical and Manufacturing Engineering
  • Sport, Exercise and Health Sciences

Published in

Biotechnology and Bioengineering

Volume

116

Issue

9

Pages

2364 - 2376

Citation

WRAGG, N.M. ... et al, 2019. Development of tissue-engineered skeletal muscle manufacturing variables. Biotechnology and Bioengineering, 116 (9), pp.2364-2376.

Publisher

© Wiley

Version

  • AM (Accepted Manuscript)

Publisher statement

This is the peer reviewed version of the following article: WRAGG, N.M. ... et al, 2019. Development of tissue-engineered skeletal muscle manufacturing variables. Biotechnology and Bioengineering, 116 (9), pp.2364-2376, which has been published in final form at https://doi.org/10.1002/bit.27074. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.

Acceptance date

2019-05-21

Publication date

2019-05-27

Copyright date

2019

ISSN

0006-3592

eISSN

1097-0290

Language

  • en

Usage metrics

    Loughborough Publications

    Exports

    RefWorks
    BibTeX
    Ref. manager
    Endnote
    DataCite
    NLM
    DC