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Title: Physiological effects of the addition of n-dodecane as an oxygen vector during steady-state Bacillus licheniformis thermophillic fermentations perturbed by a starvation period or a glucose pulse
Authors: Lopes da Silva, Teresa
Reis, Alberto
Roseiro, J. Carlos
Hewitt, Christopher J.
Keywords: Bacillis licheniformis
N-dodecane
Multi-parameter flow cytometry
Oxygen vector
Steady-state Thermophilic
Wastewater treatment
Issue Date: 2008
Publisher: © Elsevier
Citation: LOPES DA SILVA T ... et al, 2008. Physiological effects of the addition of n-dodecane as an oxygen vector during steady-state Bacillus licheniformis thermophillic fermentations perturbed by a starvation period or a glucose pulse. Biochemical Engineering Journal, 42 (3), pp. 208-216
Abstract: The effect of the presence of n-dodecane as a potential oxygen vector during oxygen limited continuous cultures of a Bacillus strain was studied, under extreme nutrient supply conditions: glucose excess, limitation and starvation. The addition of n-dodecane to the aqueous phase of a mechanically agitated and aerated fermentation increased the kLa by up to 35%. The n-dodecane additions to B. licheniformis cells during starvation (oxygen limitation with concomitant glucose starvation) caused a severe detrimental progressive change in cell physiological state with respect to cytoplasmic membrane polarisation and permeability which was mitigated against by alleviating either the oxygen limitation (by increasing the mean energy dissipation rate or by the addition of n-dodecane as an oxygen vector) or by alleviating the carbon limitation (by resuming the carbon feed or by the addition of a glucose pulse). Further that during periods of excess glucose (glucose pulse) a much higher kLa was required to prevent the onset of anaerobic mixed acid fermentation than could be provided by the addition of n-dodecane alone. N-dodecane can be used to increase the kLa when added in sufficient quantities to the aqueous phase of a mechanically agitated and aerated bioreactor but the magnitude of this increase is process and vessel geometry specific.
Description: This article was published in the journal, Biochemical Engineering Journal [© Elsevier] and the definitive version is available at: www.elsevier.com/locate/bej
Version: Accepted for publication
DOI: 10.1016/j.bej.2008.06.023
URI: https://dspace.lboro.ac.uk/2134/4082
ISSN: 1369-703X
Appears in Collections:Published Articles (Chemical Engineering)

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