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Title: High temperature liquid chromatography of steroids on a bonded hybrid column
Authors: Al-Khateeb, Lateefa A.
Smith, Roger M.
Keywords: High temperature liquid chromatography
Superheated water
Hybrid stationary phase
XTerra MS C18 stationary phase
Issue Date: 2009
Publisher: © Springer Verlag
Citation: AL-KHATEEB, L.A. and SMITH, R.M., 2009. High temperature liquid chromatography of steroids on a bonded hybrid column. Analytical and Bioanalytical Chemistry, 394 (5), pp. 1255–1260
Abstract: A hybrid stationary phase, XTerra MS C18, has been evaluated for the high temperature reversed phase liquid chromatography of selected hydrophobic steroids. The effects on the retention and efficiency at temperatures up to 130 °C and eluent compositions from methanol-water mixtures to superheated water were studied. The thermodynamic data of the separations were determined. It was shown that increasing the temperature enabled the percentage of methanol to be reduced. High mobile phase flow rates could be used but for these non-polar analytes the retention times with superheated water as the eluent were still high.
Description: This article was acepted for publication in the journal, Analytical and Bioanalytical Chemistry [© Springer-Verlag] and will be available at: http://www.springer.com/chemistry/analytical/journal/216
Version: Accepted for publication
DOI: 10.1007/s00216-009-2602-6
URI: https://dspace.lboro.ac.uk/2134/4091
ISSN: 1618-2642
Appears in Collections:Published Articles (Chemistry)

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