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Please use this identifier to cite or link to this item: https://dspace.lboro.ac.uk/2134/4240

Title: Automated, scalable culture of human embryonic stem cells in feeder free conditions
Authors: Thomas, Robert James
Anderson, David
Chandra, Amit
Smith, Nigel M.
Young, Lorraine E.
Williams, David J.
Denning, Chris
Keywords: Human embryonic stem cells
Automation
CompacT SelecT
Scalability
Process control
Issue Date: 2009
Publisher: © Wiley Periodicals
Citation: THOMAS, R.J. ... et al, 2009. Automated, scalable culture of human embryonic stem cells in feeder-free conditions. Biotechnology and Bioengineering, 102 (6), pp. 1636-1644
Abstract: Large-scale manufacture of human embryonic stem cells (hESCs) is prerequisite to their widespread use in biomedical applications. However, current hESC culture strategies are labor-intensive and employ highly variable processes, presenting challenges for scaled production and commercial development. Here we demonstrate that passaging of the hESC lines, HUES7, and NOTT1, with trypsin in feeder-free conditions, is compatible with complete automation on the CompacT SelecT, a commercially available and industrially relevant robotic platform. Pluripotency was successfully retained, as evidenced by consistent proliferation during serial passage, expression of stem cell markers (OCT4, NANOG, TRA1-81, and SSEA-4), stable karyotype, and multi-germlayer differentiation in vitro, including to pharmacologically responsive cardiomyocytes. Automation of hESC culture will expedite cell-use in clinical, scientific, and industrial applications.
Description: This article is Restricted Access. It was published in the journal, Biotechnology and Bioengineering [© Wiley] and is available at: http://www3.interscience.wiley.com/journal/117933915/grouphome/home.html
Version: Restricted access
DOI: 10.1002/bit.22187
URI: https://dspace.lboro.ac.uk/2134/4240
ISSN: 0006-3592
Appears in Collections:Closed Access (Mechanical and Manufacturing Engineering)

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