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|Title: ||Automated, scalable culture of human embryonic stem cells in feeder free conditions|
|Authors: ||Thomas, Robert James|
Smith, Nigel M.
Young, Lorraine E.
Williams, David J.
|Keywords: ||Human embryonic stem cells|
|Issue Date: ||2009|
|Publisher: ||© Wiley Periodicals|
|Citation: ||THOMAS, R.J. ... et al, 2009. Automated, scalable culture of human embryonic stem cells in feeder-free conditions. Biotechnology and Bioengineering, 102 (6), pp. 1636-1644|
|Abstract: ||Large-scale manufacture of human embryonic stem cells (hESCs) is prerequisite to their widespread use in biomedical applications. However, current hESC culture strategies are labor-intensive and employ highly variable processes, presenting challenges for scaled production and commercial development. Here we demonstrate that passaging of the hESC lines, HUES7, and NOTT1, with trypsin in feeder-free conditions, is compatible with complete automation on the CompacT SelecT, a commercially available and industrially relevant robotic platform. Pluripotency was successfully retained, as evidenced by consistent proliferation during serial passage, expression of stem cell markers (OCT4, NANOG, TRA1-81, and SSEA-4), stable karyotype, and multi-germlayer differentiation in vitro, including to pharmacologically responsive cardiomyocytes. Automation of hESC culture will expedite cell-use in clinical, scientific, and industrial applications.|
|Description: ||This article is Restricted Access. It was published in the journal, Biotechnology and Bioengineering [© Wiley] and is available at: http://www3.interscience.wiley.com/journal/117933915/grouphome/home.html|
|Version: ||Restricted access|
|Appears in Collections:||Closed Access (Mechanical and Manufacturing Engineering)|
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