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|Title: ||Processing of collagen gels to create in vitro cell growth matrix without damage to the collagen native structure|
|Authors: ||Liu, Yang|
Williams, David J.
|Keywords: ||Cell culture matrix|
|Issue Date: ||2006|
|Publisher: ||Professional Engineering Publishing / © IMechE|
|Citation: ||LIU, Y. and WILLIAMS, D.J., 2006. Processing of collagen gels to create in vitro cell growth matrix without damage to the collagen native structure. Proceedings of the Institution of Mechanical Engineers, Part B: Journal of Engineering Manufacture, 220 (5), pp. 787-791|
|Abstract: ||This preliminary work explores a technique for processing collagen gels to provide a structured matrix support for cell growth and other tissue engineering applications without using cyto-toxic photo-initiators. Collagen gels can be structured by techniques similar to those of rapid manufacturing and retain the fibril structure of native collagen. Incorporation of alpha-modified minimal essential medium (MEM) in the collagen solution improved the rate of gelation in a cell-friendly way. Local gelation of a collagen solution formulated with alpha-modified MEM can be achieved by exposure to radiation from a remote incandescent lamp source indicating that it may be possible to prepare structured gels by lithographically based rapid manufacturing processes. Exposure of the alpha-modified MEM collagen solution to the radiation also increased the thickness of the collagen fibrils formed during the gelation process to create a more structured gel. Methyl blue staining, scanning electron microscope (SEM), and differential scanning calorimetry (DSC) experiments confirmed the collagen was not denatured, i.e. the native structure of collagen was retained.|
|Description: ||This is an article from the journal, Proceedings of the IMechE, Part B: Journal of Engineering Manufacture [© IMechE]. It is also available at: http://journals.pepublishing.com/content/119784/?p=9d2d6d3454934a77b53fb65919dc3fba&pi=0|
|Appears in Collections:||Published Articles (Mechanical and Manufacturing Engineering)|
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