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Please use this identifier to cite or link to this item: https://dspace.lboro.ac.uk/2134/5761

Title: Induction studies with Escherichia coli expressing recombinant interleukin-13 using multi-parameter flow cytometry
Authors: Shitu, Jennifer O.
Woodley, John M.
Wnek, Richard
Chartrain, Michel J.
Hewitt, Christopher J.
Keywords: Cytoplasmic membrane potential
Escherichia coli
Flow cytometry
Interleukin 13
Recombinant protein
Issue Date: 2009
Publisher: © Springer Verlag
Citation: SHITU, J.O. ... et al, 2009. Induction studies with Escherichia coli expressing recombinant interleukin-13 using multi-parameter flow cytometry. Biotechnology Letters, 31 (4), pp. 577-584.
Abstract: The expression of interleukin -13 following induction with IPTG in E. coli results in metabolic changes as indicated by multi-parameter flow cytometry and traditional methods of fermentation profiling (O2 uptake rate, CO2 evolution rate and optical density measurements). Induction early in the rapid growth phase was optimal although this led to lower overall biomass concentrations and lower maximum specific growth rates. In contrast, induction in the mid-rapid growth phase was the most detrimental to cell quality as measured by cytoplamsic membrane depolarisation.
Description: This article was published in the journal, Biotechnology Letters [© Springer Verlag] and the original publication is available at www.springerlink.com
Version: Accepted for publication
DOI: 10.1007/s10529-008-9908-x
URI: https://dspace.lboro.ac.uk/2134/5761
ISSN: 0141-5492
1573-6776
Appears in Collections:Published Articles (Chemical Engineering)

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