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|Title: ||Reduction and degradation of amyloid aggregates by a pulsed radio-frequency cold atmospheric plasma jet|
|Authors: ||Bayliss, Danny|
Walsh, James L.
Kong, Michael G.
|Issue Date: ||2009|
|Publisher: ||© IOP Publishing Ltd and Deutsche Physikalische Gesellschaft|
|Citation: ||BAYLISS, D.... et al., 2009. Reduction and degradation of amyloid aggregates by a pulsed radio-frequency cold atmospheric plasma jet. New Journal of Physics, 11(115024), 16pp.|
|Abstract: ||Surface-borne amyloid aggregates with mature fibrils are used as a
non-infectious prion model to evaluate cold atmospheric plasmas (CAPs) as a
prion inactivation strategy. Using a helium–oxygen CAP jet with pulsed radiofrequency
(RF) excitation, amyloid aggregates deposited on freshly cleaved
mica discs are reduced substantially leaving only a few spherical fragments
of sub-micrometer sizes in areas directly treated by the CAP jet. Outside the
light-emitting part of the CAP jet, plasma treatment results in a ‘skeleton’
of much reduced amyloid stacks with clear evidence of fibril fragmentation.
Analysis of possible plasma species and the physical configuration of the
jet–sample interaction suggests that the skeleton structures observed are unlikely
to have arisen as a result of physical forces of detachment, but instead
by progressive diffusion of oxidizing plasma species into porous amyloid
aggregates. Composition of chemical bonds of this reduced amyloid sample
is very different from that of intact amyloid aggregates. These suggest the
possibility of on-site degradation by CAP treatment with little possibility of
spreading contamination elsewhere, thus offering a new reaction chemistry
route to protein infectivity control with desirable implications for the practical
implementation of CAP-based sterilization systems.|
|Description: ||This article was published in the journal, New Journal of Physics, [© IOP Publishing Ltd and Deutsche Physikalische Gesellschaft] and is also available at: http://dx.doi.org/10.1088/1367-2630/11/11/115024|
|Appears in Collections:||Published Articles (Electronic, Electrical and Systems Engineering)|
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