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Please use this identifier to cite or link to this item: https://dspace.lboro.ac.uk/2134/7017

Title: An investigation into the preservation of microbial cell banks for alpha-amylase production during 5L fed-batch Bacillus licheniformis fermentations
Authors: Hancocks, Nichola H.
Thomas, Colin R.
Stocks, Stuart M.
Hewitt, Christopher J.
Keywords: Bacteria
Flow cytometry
Membrane potential
Membrane integrity
Cryopreservation
Fermentation
Bacillus licheniformis
Issue Date: 2010
Publisher: © Springer
Citation: HANCOCKS, N.H. ... et al, 2010. An investigation into the preservation of microbial cell banks for alpha-amylase production during 5L fed-batch Bacillus licheniformis fermentations. Biotechnology Letters, 32 (10), pp. 1405-1412.
Abstract: Fluorescent staining techniques were used for a systematic examination of methods used to cryopreserve microbial cell banks. The aim of cryopreservation here is to ensure subsequent reproducible fermentation performance rather than just post thaw viability. Bacillus licheniformis cell physiology post-thaw is dependent on the cryopreservant (either Tween 80, glycerol or dimethyl sulphoxide) and whilst this had a profound effect on the length of the lag phase, during subsequent 5 l fed-batch fermentations, it had little effect on maximum specific growth rate, final biomass concentration or a-amylase activity. Tween 80 not only protected the cells during freezing but also helped them recover post-thaw resulting in shorter process times.
Description: The final publication is available at www.springerlink.com
Version: Accepted for publication
DOI: 10.1007/s10529-010-0308-7
URI: https://dspace.lboro.ac.uk/2134/7017
Publisher Link: http://dx.doi.org/10.1007/s10529-010-0308-7
ISSN: 0141-5492
Appears in Collections:Published Articles (Chemical Engineering)

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