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Please use this identifier to cite or link to this item: https://dspace.lboro.ac.uk/2134/8617

Title: Multi-parameter flow cytometry and cell sorting reveal extensive physiological heterogeneity in Bacillus cereus batch cultures.
Authors: Want, Andrew
Hancocks, Helen
Stocks, Stuart M.
Nebe-von-Caron, Gerhard
Hewitt, Christopher J.
Keywords: Bacillus cereus
Cell sorting
Bis-(1,3-dibutylbarbituric acid) trimethine oxonol
3,30-Dihexylocarbocyanine iodide
Flow cytometry
Membrane potential
Membrane integrity
Issue Date: 2011
Publisher: © Springer
Citation: WANT, A. ... et al, 2011. Multi-parameter flow cytometry and cell sorting reveal extensive physiological heterogeneity in Bacillus cereus batch cultures. Biotechnology Letters, 33 (7), pp. 1395–1405.
Abstract: Based on two staining protocols, DiOC6(3)/ propidium iodide (PI) and RedoxSensor Green (an indicator of bacterial reductase activity)/PI, multiparameter flow cytometry and cell sorting has identified at least four distinguishable physiological states during batch cultures of Bacillus cereus. Furthermore, dependent on the position in the growth curve, single cells gave rise to varying numbers of colonies when sorted individually onto nutrient agar plates. These growing colonies derived from a single cell had widely different lag phases, inferred fromdifferences in colony size.This further highlights the complex population dynamics of bacterial monocultures and further demonstrates that individual bacterial cells in a culture respond in markedly dissimilar ways to the environment, resulting in a physiologically heterogenous and dynamic population.
Description: The final publication is available at http://www.springerlink.com
Version: Accepted for publication
DOI: 10.1007/s10529-011-0566-z
URI: https://dspace.lboro.ac.uk/2134/8617
Publisher Link: http://dx.doi.org/10.1007/s10529-011-0566-z
ISSN: 0141-5492
Appears in Collections:Published Articles (Chemical Engineering)

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