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Title: Determination of free desmosine and isodesmosine as urinary biomarkers of lung disorder by ultra performance liquid chromatography-ion mobility-mass spectrometry
Authors: Devenport, Neil A.
Reynolds, James C.
Parkash, Ved
Cook, Jason
Weston, Daniel J.
Creaser, Colin S.
Keywords: Ion mobility-mass spectrometry
Urinary analysis
COPD
Desmosine
Isodesmosine
Issue Date: 2011
Publisher: © Elsevier
Citation: DEVENPORT, N.A. ... et al, 2011. Determination of free desmosine and isodesmosine as urinary biomarkers of lung disorder by ultra performance liquid chromatography-ion mobility-mass spectrometry. Journal of Chromatography B, 879 (32), pp. 3797-3801
Abstract: The elastin degradation products, desmosine (DES) and isodesmosine (IDES) are highly stable, cross-linking amino-acids that are unique to mature elastin. The excretion of DES/IDES in urine, in the free form and with associated peptide fragments, provides an indicator of lung damage in chronic obstructive pulmonary disease (COPD). A quantitative ion mobility-mass spectrometry (IM-MS) method has been developed for the analysis of free DES/IDES in urine with deuterated IDES as an internal standard. Resolution of DES/IDES isomers was achieved in less than five minutes using ultra performance liquid chromatography (UPLC) combined with ion pairing. The optimized UPLC-IM-MS method provided a linear dynamic range of 10-300 ng/mL and a limit of quantitation of 0.028 ng/mL for IDES and 0.03 ng/mL for DES (0.55 ng and 0.61 ng on column respectively). The method reproducibility (%RSD) was < 4% for DES and IDES. The UPLC-IM-MS method was applied to the analysis of urine samples obtained from healthy volunteers and COPD patients. The DES/IDES concentrations in healthy and COPD urine showed an increase in DES (79%) and IDES (74%) in the COPD samples, relative to healthy controls. The incorporation of an IM separation prior to m/z measurement by MS was shown to reduce non-target ion responses from the bio-fluid matrix.
Description: This article was published in the serial, Journal of Chromatography B [© Elsevier]. The definitive version is available at: http://www.sciencedirect.com/science/article/pii/S1570023211006817
Version: Accepted for publication
DOI: 10.1016/j.jchromb.2011.10.016
URI: https://dspace.lboro.ac.uk/2134/8992
Publisher Link: http://www.sciencedirect.com/science/article/pii/S1570023211006817
ISSN: 1570-0232
Appears in Collections:Published Articles (Chemistry)

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