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|Title: ||Determination of free desmosine and isodesmosine as urinary biomarkers of lung disorder by ultra performance liquid chromatography-ion mobility-mass spectrometry|
|Authors: ||Devenport, Neil A.|
Reynolds, James C.
Weston, Daniel J.
Creaser, Colin S.
|Keywords: ||Ion mobility-mass spectrometry|
|Issue Date: ||2011|
|Publisher: ||© Elsevier|
|Citation: ||DEVENPORT, N.A. ... et al, 2011. Determination of free desmosine and isodesmosine as urinary biomarkers of lung disorder by ultra performance liquid chromatography-ion mobility-mass spectrometry. Journal of Chromatography B, 879 (32), pp. 3797-3801|
|Abstract: ||The elastin degradation products, desmosine (DES) and isodesmosine (IDES) are highly
stable, cross-linking amino-acids that are unique to mature elastin. The excretion of
DES/IDES in urine, in the free form and with associated peptide fragments, provides an
indicator of lung damage in chronic obstructive pulmonary disease (COPD). A quantitative
ion mobility-mass spectrometry (IM-MS) method has been developed for the analysis of free
DES/IDES in urine with deuterated IDES as an internal standard.
Resolution of DES/IDES isomers was achieved in less than five minutes using ultra
performance liquid chromatography (UPLC) combined with ion pairing. The optimized
UPLC-IM-MS method provided a linear dynamic range of 10-300 ng/mL and a limit of
quantitation of 0.028 ng/mL for IDES and 0.03 ng/mL for DES (0.55 ng and 0.61 ng on
column respectively). The method reproducibility (%RSD) was < 4% for DES and IDES.
The UPLC-IM-MS method was applied to the analysis of urine samples obtained from
healthy volunteers and COPD patients. The DES/IDES concentrations in healthy and
COPD urine showed an increase in DES (79%) and IDES (74%) in the COPD samples,
relative to healthy controls. The incorporation of an IM separation prior to m/z measurement
by MS was shown to reduce non-target ion responses from the bio-fluid matrix.|
|Description: ||This article was published in the serial, Journal of Chromatography B [© Elsevier]. The definitive version is available at: http://www.sciencedirect.com/science/article/pii/S1570023211006817|
|Version: ||Accepted for publication|
|Publisher Link: ||http://www.sciencedirect.com/science/article/pii/S1570023211006817|
|Appears in Collections:||Published Articles (Chemistry)|
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