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Title: Determination of inflammatory and prominent proteomic changes in plasma and adipose tissue after high intensity intermittent training in overweight and obese males
Authors: Leggate, Melanie
Carter, Wayne
Evans, Matthew J.C.
Vennard, Rebecca A.
Sribala-Sundaram, Sarah
Nimmo, Myra A.
Keywords: Exercise
Training
Obesity
Cytokines
Adipose tissue proteomic
Slow-grade inflammation
Issue Date: 2012
Publisher: © American Physiological Society
Citation: LEGGATE, M. ... et al., 2012. Determination of inflammatory and prominent proteomic changes in plasma and adipose tissue after high intensity intermittent training in overweight and obese males. Journal of Applied Physiology, 112 (8), pp. 1353 - 1360
Abstract: This study aimed to determine whether 2 wk of high-intensity intermittent training (HIIT) altered inflammatory status in plasma and adipose tissue in overweight and obese males. Twelve participants [mean (SD): age 23.7 (5.2) yr, body mass 91.0 (8.0) kg, body mass index 29.1 (3.1) kg/m2] undertook six HIIT sessions over 2 wk. Resting blood and subcutaneous abdominal adipose tissue samples were collected and insulin sensitivity determined, pre- and posttraining. Inflammatory proteins were quantified in plasma and adipose tissue. There was a significant decrease in soluble interleukin-6 receptor (sIL-6R; P = 0.050), monocyte chemotactic protein-1 (MCP-1, P = 0.047), and adiponectin (P = 0.041) in plasma posttraining. Plasma IL-6, intercellular adhesion molecule-1 (ICAM-1), tumor necrosis factor-α (TNF-α), IL-10, and insulin sensitivity did not change. In adipose tissue, IL-6 significantly decreased (P = 0.036) and IL-6R increased (P = 0.037), while adiponectin tended to decrease (P = 0.056), with no change in ICAM-1 posttraining. TNF-α, MCP-1, and IL-10 were not detectable in adipose tissue. Adipose tissue homogenates were then resolved using one-dimensional gel electrophoresis, and major changes in the adipose tissue proteome, as a consequence of HIIT, were evaluated. This proteomic approach identified significant reductions in annexin A2 (P = 0.046) and fatty acid synthase (P = 0.016) as a response to HIIT. The present investigation suggests 2 wk of HIIT is sufficient to induce beneficial alterations in the resting inflammatory profile and adipose tissue proteome of an overweight and obese male cohort.
Description: This article is closed access. It was published in the Journal of Applied Physiology [© the American Physiological Society] and the definitive version is available at: http://dx.doi.org/10.​1152/​japplphysiol.​01080.​2011
Version: Accepted for publication
DOI: 10.​1152/​japplphysiol.​01080.​2011
URI: https://dspace.lboro.ac.uk/2134/9709
Publisher Link: http://jap.physiology.org/content/112/8/1353
ISSN: 0021-8987
Appears in Collections:Closed Access (Sport, Exercise and Health Sciences)

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